Pune
Fertility Center Cryobank History.
The PFC Cryobank is
a full service sperm bank founded in May 2001. It has been
providing for physician and patients an accessible, convenient
and comprehensive resource for semen cryopreservation and
specialized reproductive techniques.
We are proud of our record of reproductive success with cryopreserved
specimens.
We constantly aim for stringest Quality & Service.
Our Services
:
Freezing Donor semen storage.
The freezing & storing of human sperm for use in artifical
insemination AID.
Long term Semen storage.
For men facing the possibility of sterilization, reduction
in fertilizing potential or
any genetic damage due to chemotherapy, radiation therapy,
high risk occupational exposures, vasectomy.
Samples of husbands who are serving in army & those who
will be away, when their wives are undergoing stimulation
for IVF procedures . For men who have difficulty in producing
sample on demand especially those couples undergoing IVF.
An Historical perspective
of Sperm Banking.
The prefix “cryo”
derived from Greek word “Kyros” which means Frost
or cold.
The father of Artificial insemination Spallanzani, marked
up another first in reproductive biology. It is believed that
he was the first to report the effects of cooling on human
sperm in 1776, his observation was that sperms cooled by snow
became motionless.
It wasnot until the mid 1800’s that effort to freeze
sperm began.
In 1866 Montegazza was the first to envision banks for frozen
human sperm. His suggestion “ a man dying on the battle
field may beget a legal heir with his semen frozen & stored
at home.” It took 150 years, during the Gulf war crisis
in 1992. Montegazza’s vision became a reality. Service
men were able, indeed many opted to freeze & store specimens
for their sperm before leaving for battle. During year 1938-1945
numerous scientists made observations of sperm ability to
survive freezing & storage temperature as low as minus
321 fahrenheit. But surviving is alone not accepted in ART,
but being able to successfully function in the conception
process is also important.
First major break through in this area came in 1949 when A
S Parker & two British scientist developed a syrupy substance
- k/a Glycerol to protect semen from injury during freezing
in 1953. Dr. Jerome.K.Sherman an American pioneer in sperm
freezing , further refined this process. He introduced a simple
process of preserving human sperm using glycerol , but this
was combined with slow cooling of sperm and storage using
carbon dioxide as a refrigerant .
He also for the first time demonstrated that frozen sperm
when thawed were able to fertilize an egg, induce its normal
development . First successfull human pregnancy with frozen
spermetoza was reported in 1953 . The 11th International conference
of genetics in 1963 triggered interest in the possibility
of sperms banks.
A decade later in early 70’s the first commercial sperm
bank opened . Sperm banks in the
eighties , focused their main aim on the increasing demand
for anonymous donor insemination .
Frozen sperm
becomes the Gold Standard
In 1985, a dramatic
event hastened the transition to predominate use of frozen
sperm for DI , the identification of the deadly devastating
newly recognized sexually transmitted disease AIDS.
In Feb 1988 American fertility now renamed as America Society
for Reproductive medicine . The food & Drug Administration
and the CDC Center for disease control recommended that only
frozen semen to be used for Donor ‘insemination’
in conjunction with a minimum of 6 months quaratine period
.
Thus the scientific community realized the best way to ensure
semen not infected
With HIV, hepatitis & other sexually transmitted diseases
is to freeze & quarantine the specimen for 6 months, after
which the donor is retested for above tests. Thus reducing
the possibility that the donor had the virus at the time the
specimen was collected & frozen. Today, all sperm's
used for Donor Insemination are frozen and quarantined semen
samples. Thus sperm Banks have a vital role in reproductive
biology.
The ability to freeze to store sperms has contributed &
played on integral part in the development of today’s
more effective reproductive technologies.
Cryobank Donor
Criteria :
We follow stringent
screening process before a male semen sample is selected in
our donor Programe strictly following all the international
selection guideline protocols.
The process starts
:
A phone call : begins the process – our donor coordinator
receives the calls of potential donors. A few basic questions
are asked after which they are scheduled for a personal appointment.
During the course of personal interview basic questions about
their height, weight, age and educational background are noted.
Donor under 18 are not accepted & the upper age limit
is 45 years. Our cryobank requires donors who are students
undergoing basic gradute course or graduates.
Those men are accepted who can provide a detailed medical
information. Additional genetic information if available is
always welcome. The Medical information includes theirs as
well as their family members. Questions pertaining to specific
medical condition such as cancer, diabetics any genetic disorder
begin the screening process.
The application process : starts once the potential donor
meets the above listed requirements. He completes the donor
application form . Next he is scheduled an appointment to
provide a semen sample for analysis to determine if the donor
semen
meets our standards . Once this criteria is fulfilled, he
takes a short personality quiz, undergoes a physical examination
& various laboratory test .
The Lab tests performed during initial screening of cryobank
donors includes
· ABO-Rh blood typing
· Bio-chemistry panel
· Complete Blood Count
· Semen Analysis
· Urine Analysis
Following investigation carried out during initial screening
and repeated every 3 months till then specimen is quarantined
for 90 days & not released until the donor has minimum
2 negative HIV screening.
1. SGPT
2. Hepatitis B surface Antigen
3. HIV antibody tests
4. RPR (for syphilis)
A potential donor must meet certain requirements in order
to qualify for our donor program. The sperm count must be
atleast 75 million sperm per cc, with 70% motility with 60%
having normal morphology.
Our Counsellers conducts
a Medical interview, in which review of the long profile completed
by each donor. Information about health of a donor her siblings,
his parents, his grandparents his maternal uncles & aunts,
paternal uncles, aunts & his cousins. The Medical and
family history is carefully evaluated for any birth defect
or known genetic condition. A donor is not accepted in the
donor program if there is a family history which indicates
that his offspring may be at increased risk for a birth or
a known genetic condition.
Why should one consider
semen storage?
The rapid advances
being made in fields of reproductive biology, urology &
oncology is providing men facing with possible sterilization
new options regarding the preservation of their reproductive
capabilities. Hence these male patients may want to consider
the option of semen storage prior to proceeding with any medical
procedure that may result in the loss of fertility potential.
Semen Storage should be considered
A) Prior to Cancer
related treatment :
Advances in the Cancer therapy in many types of cancer has
improved the prognosis for many cancer therapy in many types
of cancer has improved the But at the same time their therapies
frequently render the male patients infertile. prognosis for
many cancer patients. Semen cryopreservation prior to such
therapies initiation offers a possibility of retaining reproductive
capabilities for male patients.
B) Prior to testicular or Prostrate surgery or prostatechtomy
C) Prior to Vasectomy
D) Prior to IVF, GIFT, ZIFT procedures ensures the semen backup.
E) For High risk occupational exposures.
In recent times it is seen on the job exposure to hazardous
materials can have significant health consequences includes
male infertility. High risk occupational exposures include
but not limited to these are environmental toxins exposure,
like chemicals, herbicides, pesticides, radiation etc.
F) Man in Military facing life threatening situations avail
cryo preservation techniques.
G) Professional atheletes who are at risk for testicular
injury.
H) At times of traveling by Husbands.
Husbands schedule doesn’t permit his availability at
the time of the treatment schedule the cryo preserved semen
allows wife to continue, with couple's reproductive plan.
I) For Oligo spermic man.
Pooling of previously banked semen specimen in male who is
diagnosed oligo spermic and using this pooled and prepared
sample, for one insemination increases the chance of pregnancy.
|